Growth and Vegetative Development
Chang, Yu-Yun , Kao, Chai-Wei , Yang, Chang-Hsien .
Functional Analysis and Characterization of GIGANTEA (GI) and CONSTANS (CO)-like Orthologues from Orchid (Oncidium Gower Ramsey).
Orchids are among the most important plants in flower markets around the world. No research on the characterization of flowering time genes or flower transition has been reported in orchids. We report here on the isolation and characterization of orthologue of flowering time gene GIGANTEA (GI) and CONSTANS (CO)-like genes from orchid (Oncidium Gower Ramsey). OnGI cDNA encoded an 1160 amino acids protein and showed 72% identity to rice OsGI. OnGI mRNA is expressed throughout development and can be detected in various organs such as leaves, pseudobuds, shoot, root and flowers of O. Gower Ramsey. The expression level of OnGI mRNA varied under LD conditions and cycled vigorously, with the level highest at the 8 to 12 h of the light period and lowest at dawn. Ectopic expression of OnGI promoted flowering time in wild type and rescued the late-flowering phenotype in the gi mutant of Arabidopsis. OnCOL1 encodes a 328 amino acid protein. Phylogenetic analysis demonstrated that OnCOL1 is most similar to Arabidopsis CO-like gene AtCOL5. OnCOL1 mRNA was expressed throughout development and can be detected in various organs such as root, inflorescence, leaves, and flowers of Oncidium. Similar to Arabidopsis CO mRNA, the expression levels of OnCOL1 mRNA varied under LD conditions and cycled vigorously. Functional analysis of OnCOL1 through transgenic approach is in progress.
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1 - National Chung Hsing University, Graduate Institute of Biotechnology, Taichung, 40227, Taiwan (R.O.C.)
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM