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Abstract Detail


Protein Targeting and Vesicular Trafficking

Tse, Yu Chung [1], Liu, Sin Ting [1], Jiang, Liwen [1].

Biogenesis of Protein Storage Vacuoles (PSVs) in Tobacco Developing Seeds.

Storage proteins accumulate in protein storage vacuoles (PSVs) during seed development. PSVs in most dicotyledons contain three morphologically distinct subcompartments: crystalloid, matrix and globoid. Recent studies demonstrated that the formation of PSV and its subcompartments were developmentally regulated during seed development. Here we use developing tobacco seeds as a model to study PSV biogenesis during seed development. Towards this goal, we have studied the morphological changes of developing PSVs via structural transmission electron microscopy and defined developing PSVs by PSV tonoplast marker alpha-TIP (tonoplast intrinsic protein). Using structural TEM, we have also illustrated the morphology of various transport vesicles or prevacuolar compartments (PVCs) likely involved in the formation of PSV subcompartments during seed development. Using specific organelle markers as probes in immunogold EM, we have defined the membrane origins of these transport vesicles or PVCs in developing tobacco seeds. Further studies will be carried out to define the function of these PVCs and illustrate the uptake or fusion process of PVCs into developing PSVs. Supported by grants from the Research Grants Council of Hong Kong (CUHK4260/02M, CUHK4307/03M and CUHK4580/05M), National Science Foundation of China (30529001), CUHK Scheme C and UGC-AoE (B-07/99) to L. Jiang.


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1 - The Chinese University of Hong Kong, Department of Biology

Keywords:
Protein storage vacuole
Prevacuolar compartments.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P18005
Abstract ID:157


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