Jeong, Jin Seo , Cheong, Pil-Joong , Baek, Kwang-Hun , Park, young Taik , Kim, Yeon-Ki , Kim, Ju-Kon .
Rice NACs in Stress- and Yield-Related Traits.
Many environmental stimuli including water potential, temperature extremes, and high salinity, regulate gene expression at transcriptional and post-transcriptional levels. Expression profiling was conducted with RNAs extracted from rice leaves grown in the time course of stress treatments using the 60K Rice Whole Genome Microarray. A number of genes encoding transcription factors with NAC domain were found to be up-regulated by stress treatments. NAC protein family comprises a variety of plant proteins that are identified by the presence of a highly conserved N-terminal NAC domain. Transgenic overexpression of these genes in rice plants under the control of either a constitutive or a root-specific promoter resulted in enhanced tolerance to the stresses. Interestingly, in some of the NAC transgenic plants, significant increases in yield-related parameters were observed as well. These parameters include leaf biomass, emergence vigour, thousand kernel weight, panicles, and total number of seeds. By combining results from the expression profiling between NAC overexpressors and nontransgenic controls and those from chromatin immunoprecipitation followed by promoter microarray analysis, we identified target genes that were activated by the NAC proteins. We conclude that NAC proteins activate the target genes, which not only make the transgenic plants tolerant to abiotic stresses but also increase their yield.
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1 - Myongji University, School of Biotechnology and Environmental Engineering, San 38-2, Nam-dong, Yongin, Kyonggi-do, 449-728, Korea
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM