Ramaiah, Madhuvanthi , Karthikeyan, Athikkattuvalasu S , Raghothama, Kashchandra G .
Glycerol-3-phosphate permease, a putative organic phosphate transporter and its potential role during phosphate stress in plants.
Phosphate (Pi) deficiency causes drastic changes in several plant metabolic processes like photosynthesis, respiration, carbon metabolism and energy balance. As a response to Pi stress plants have evolved numerous adaptive mechanisms. Phosphate transporters are one of the major determinants of this Pi starvation response in plants. The transporters involved in the movement of C3 and C6 compounds play an important role in the metabolic adaptations during Pi stress. The current study aims to characterize Glycerol-3-phosphate permease, a sugar phosphate transporter which is regulated by Pi stress in plants. This transporter belongs to the Major Facilitator Superfamily and occurs as a gene family of 5 members in Arabidopsis (AtPS3 gene family). Transcript analysis and characterization of promoter-reporter gene fusions shows that the members of this gene family have differential spatio-temporal regulation during Pi stress. The expression of AtPS3;1, AtPS3;4 and AtPS3;5 is found in all parts of the plant while AtPS3;2 and AtPS3;3 are expressed specifically in the roots. The homolog of AtPS3 has been identified in tomato (LePS3) and is induced by Pi stress. The function of AtPS3 is being determined by using knock out mutants, over-expressing transgenics and bacterial complementation studies. Glycerol-3-phosphate helps in maintenance of redox balance within the cell and in the biosynthesis of galactolipids which replace phospholipids in membranes during Pi stress. The regulation of a putative transporter for this C3 compound by Pi stress, indicates the necessity to mobilize this compound within the plant. This study will lead to a comprehensive functional analysis of Glycerol-3-phosphate permeases in Arabidopsis.
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1 - Purdue University, Department of Horticulture, 625,Agricultural Mall drive, West Lafayette, Indiana, 47906, USA
2 - Purdue University, Department of Horticulture
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM