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Abstract Detail


Plant Phylogenomics: Defining Synergies Between Plant Systematics and Genome Biology

Wessler, Susan [1].

So many transposons, so little time: using phylogenomics to guide experimental design.

An unexpected finding from the analysis of genome sequences is that transposable element (TE) content varies from species to species in two important ways: by the types of TEs present and their fractional representation in the genome, and by the level of TE activity. For example, the yeast Saccharomyces cerevisiae has only LTR retrotransposons, while non-LTR retrotransposons predominate in the genomes of primates. The genomes of flowering plants, including both monocots and dicots have a rich collection of virtually types of TEs.
Genome-wide activity of TEs also varies from species to species. Flowering plants, especially members of the grass clade are in an epoch of TE-mediated genome diversification, with the participation of diverse element types.  One goal of determining the TE landscape of entire genomes is to understand how variations in TE content from species to species may influence both the quality and quantity of genomic variation and, in this way, impact the trajectory of genome and organismal evolution. Another goal of whole genome analysis of TEs is to identify potentially active elements and attempt to reactivate them in a variety of transgenic system such as cell culture, yeast or Arabidopsis.
In this presentation, I will present an overview of the TE landscape of several plant genomes including Arabidopsis thaliana, Brassica oleracea, Lotus japonicus, Oryza sativa and Zea mays. In addition I will summarize our analyses of the TEs in the genome of rice (Oryza sativa) and how we have successfully employed computational analysis coupled with transgenic Arabidopsis and yeast to reactivate long-dormant elements in order to understand genome-wide interactions between TEs and host genes. I will also discuss how knowledge of the TE landscape of the relatively stable rice genome is informing our studies into the extremely dynamic genome of maize.


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Related Links:
Wessler laboratory site
http://daffodil.plantbio.uga.edu/~suew/


1 - University of Georgia, Dept. of Plant Biology, Athens, GA, 30602, USA

Keywords:
transposable elements
genome evolution
bioinformatics.

Presentation Type: Symposium or Colloquium Presentation
Session: SY03
Location: Stevens 2/Hilton
Date: Monday, July 9th, 2007
Time: 10:45 AM
Number: SY03006
Abstract ID:1539


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