Li, Yongqing , Hua, Jian .
Identifying natural modifiers of a copine mutant bon1bon3.
Copine proteins are highly conserved among protozoa, plants, nematodes, and mammals. The calcium-dependent lipid binding activity of these proteins suggests their potential roles in membrane trafficking and signal transduction. However, the biochemical and biological functions of these proteins are not well understood. There are three members of copines in Arabidopsis thaliana, named as BON1, BON2 and BON3. These genes have both distinct and overlapping roles in regulating growth and defense in plants. The overlapping function between BON1 and BON3 are accession dependent. bon1bon3 double mutant in the Col-0 background is seedling lethal while it is wild-type like in the Ws background. Genetic studies indicate the existence of multiple natural modifiers of the bon1bon3 phenotype, named as LCDs (Lesion Cell Death). Previous study showed that the activation of a Col-specific TIR-NBS-LRR type of resistance (R) gene SNC1, located in RPP5 R gene cluster, is responsible for the cell death phenotype and the snc1 mutation can partially rescue the lethal phenotype of bon1bon3 in Col. To uncover other LCDs besides SNC1, QTL mapping is carried out using RILs (recombinant inbred lines) generated from a cross between bon1bon3snc1 (Col) and bon1bon3(Ws). Genotyping and phenotyping data analyzed with QTL Cartographer revealed 6 loci linked to the cell death phenotype. Interestingly, all of them are linked with clustered TIR-NBS-LRR type R genes. To test whether these R genes are LCDs, loss-of-function mutations of these genes are being introduced to bon1bon3snc1 to determine whether they could partially rescue the cell death phenotype. It is likely that BON family regulates cell death and growth homeostasis through multiple TIR-NBS-LRR type R genes.
Log in to add this item to your schedule
1 - Cornell University, Plant Biology, 141 Emerson Hall, Cornell University, Ithaca, NY, 14853, USA
2 - Cornell University, Plant Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM