| Abstract Detail
Plant biotech & Risk Assessment Kim, Young Jin [1], Park, Tae Il [2], Seo, Hong Yul [3], Kim, Jung Gon [4]. Transgenic soybean with enhanced tolerance to abiotic stress in the cotyledonary node method. This study was conducted to introduce an Arabidopsis NDPK2 cDNA into soybean (Glycine max) via organogenesis using Agrobacterium tumefaciens to develop transgenic soybean plants with abiotic stress tolerance. Putative transgenic plants were selected from multiple shoots on B5 medium supplemented with 2 mg/l BAP containing 13 mg/l hygromycin B. Regenerated plantlets were developed and then transferred to a nutrient solution before transplanting to soil. Putative selected transformants were confirmed for the stable integration of the transgene into the soybean genomic DNA by PCR and Southern blot analyses. Five regenerated plantlets obtained on the selection medium from 368 cotyledons. However, only one plantlet was transformed. Transgenic plant showed about 7-11% reduction in membrane damage caused by MV, indicating the expression of AtNDPK2 transgene. The results demonstrated that the transformation system developed in this study employing the plant regeneration from cotyledonary node is applicable in transgenic soybean production with abiotic stress tolerance.(Abbreviations: NDPK2 = nucleoside diphosphate kinase 2, BAP = 6-benzyl-aminopurine, MV = methyl viologen)
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1 - Honam Agricultural Research Institute, NICS, RDA, Winter Cereal & Upland Crops, 381 Songhak-dong, Iksan, 570-080, Republic of Korea
2 - Honam Agricultural Research Institute, NICS, RDA, Winter Cereal & Upland Crops
3 - Honam Agricultural Research Institute, NICS, RDA
4 -
Keywords:
Soybean
transformation
cotyledonary node
NDPK2
abiotic and biotic stresses.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P45003
Abstract ID:153 |
