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Abstract Detail


Evolutionary Developmental Biology (Evo-Devo)

Gregorich, Michele [1], Fisher, Roxanne [2].

Characterization of Hαtube, a Ceratopteris richardii meristem mutant.

To understand the evolution of plant developmental mechanisms we need to better understand the development of seedless plants. We are studying the auxin-mediated control of lateral meristem activation in gametophytes of the fern Ceratopteris richardii. Early growth of the gametophyte is generated by an apical meristem, and female/hermaphrodites generate a second lobe by activating a lateral meristem on either side of the prothallus, while males do not activate a lateral meristem. Our studies with auxins and auxin-regulating compounds show that exogenous auxin represses lateral meristem development, auxin is necessary for limiting the initiation of a lateral meristem to one side of the prothallus, and auxin polar transport is required for the proper timing of lateral meristem initiation. In this poster we present the initial characterization of a meristem mutant, Hαtube. Instead of planar growth, female/hermaphrodite mutants exhibit rounded or tubular growth through the action of additional meristematic areas, and male gametophytes generate a second apical-basal axis and show a range of shapes form twin gametophytes to a bifurcated gametophyte. Although mutant gametophytes show an abnormal distribution of archegonia, they are still capable of fertilization and producing sporophytes. Hαtube gametophytes respond to culture on synthetic auxins in a similar fashion to wild-type gametophytes.


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1 - Chatham College, Biology, Buhl Hall, Woodland Road, Pittsburgh, PA, 15232, USA
2 - Chatham College, Biology, Woodland Road, Pittsburgh, Pennsylvania, 15232, USA

Keywords:
meristem
auxin
development and evolution
Gametophyte
Fern.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P70007
Abstract ID:1521


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