Stevenson, Lauren , Yang, Xin , Ramonell, Katrina .
Receptor-like Kinases as Negative Regulators in Arabidopsis Innate Immunity.
Innate immunity is an ancient and conserved mechanism which relies on preformed receptors to detect conserved molecular signals triggering downstream broad-spectrum defense responses. Innate immunity in plants can be triggered by a wide variety of molecules including (glyco) proteins, peptides, carbohydrates and lipids. Recently, oligosaccharide elicitors such as chitin and oligogalacturonide have received attention as important signals in plant defense responses. In order to elucidate events specific to the chitin response in Arabidopsis, we compared publicly available microarray data sets of Arabidopsis treated with Chitin, Flg22, and oligogalacturonides (OG). Several leucine-rich repeat receptor-like kinases (LRR-RLKs) were identified that responded only to chitin elicitation. We acquired Salk T-DNA insertional lines in the RLKs, screened them for homozygous insertions, and performed an initial infection experiment with powdery mildew. Interestingly, three of the lines infected showed increased resistance to powdery mildew suggesting possible roles as negative regulators of fungal innate immunity. Further characterization of these RLKs will be presented in order to determine their roles in chitin-mediated defense responses.
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1 - University of Alabama Tuscaloosa, Biological Sciences, 411 Hackberry Lane Rm 301 Biology Building, Rm 301 Biology Building, Tuscaloosa, AL, 35487, USA
2 - University of Alabama Tuscaloosa, Biological Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM