Skibbe, David , Zhang, Xin , Suzuki, Nagi , Fernandes, John , Morrow, Darren , Burlingame, Al , Walbot, Virginia .
Mutator transposition alters the proteome and transcriptome of maize anthers.
The Mutator (MuDR/Mu) transposon family of maize comprises an autonomous element (MuDR) and several types of slave elements, all of which share ~215 bp terminal inverted repeat sequences and characteristically transpose late in development. There are two outcomes of Mu transposition: cut only or cut-and-paste in strictly somatic tissues and net replicative transposition in reproductive tissue. Aside from the MuDR-encoded MURA and MURB proteins, other factors required for Mu transposition, particularly those contributing to the developmentally specific behavior, have yet to be identified. Using genomic and proteomic-based approaches, our goals are to: 1) determine whether Mutator activity changes the host proteome and 2) identify host proteins characteristic of cells where net replicative transposition is the major pathway. To investigate the effect of Mutator activity on the host proteome, protein was isolated from pre-meiotic anthers of transposon-active and epigenetically silenced (inactive) sister lines and subjected to 2D-Difference Gel Electrophoresis (2D-DIGE). Of the approximately 2,500 protein spots visualized, Mu-active and -inactive individuals exhibited 48 statistically significant differentially regulated spots. Interestingly, 47 out of the 48 spots were up-regulated in the transposon active lines. Thus far, thirty-four of the forty-seven spots have been sequenced via LC-MS/MS and determined to encode genes with mainly metabolic functions. Transcriptome profiling experiments confirmed a subset of the proteome changes and also identified additional genes that are differentially regulated between transposon active and inactive lines. Implications of these results on Mutator biology will be discussed. D.S. is a NIH Ruth L. Kirschtein Post-doctoral Fellow.
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1 - Stanford University, Department of Biological Sciences, 385 Serra Mall, Stanford, CA, 94305-5020, USA
2 - University of California, San Francisco, Department of Pharmaceutical Chemistry, Mass Spectrometry Facility, 521 Parnassus Ave, Room C-18, San Francisco, CA, 94143-0446, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM