Hwang, Byung-Ho , Kim, Shinje , Kim, Do-Sun , Lim, Myung-Ho , Park, Beom-Seok , Kim, Jongkee .
Functional Analysis and Molecular Characterization of Polygalacturonase-Inhibiting Proteins (PGIPs) in Chinese Cabbage.
The physiological function as well as molecular properties of polygalacturonase-inhibiting proteins (PGIPs) from Chinese cabbage were examined by cloning, transformation, and induction study. cDNA library screening revealed that at least 5 genes of PGIPs exist in Chinese cabbage. Among these genes, BrPGIP2 was cloned from J3-11M-54 and J3-11M-3, a relatively resistant and susceptible to bacterial soft rot, respectively. Both genotypes possess the identical open reading frame (ORF) of 999 bp long which encoded a polypeptide of 332 amino acids. However, genomic DNA showed a single 72 bp intron in the resistant line and 75 bp in the susceptible line. An estimated molecular mass of the cloned BrPGIP2 was 37.1 kD and the isoelectric point (pI) of the protein was 8.65. The putative amino acid sequence of the BrPGIP2 showed 50~75% similarity to the extra-cellular PGIPs of other plant origin. Expression of BrPGIP genes were differentially regulated by various organs as well as biotic and abiotic signals. The inoculation of Erwinia carotovora ssp. carotovora (Ecc), a bacterium causing a soft rot disease, revealed that the expression of BrPGIP2 from J3-11M-54 genotype was strongly and rapidly induced compared to J3-11M-3 line. Microarray analysis from the tissue inoculated with Ecc confirmed that the expression of BrPGIP2 gene was increased more that 20-fold at maximum during soft rot development. Modeling study provided that BrPGIP2 protein from Chinese cabbage contains 10 LRR domains, showing a typical pathogen-related protein, and a right handed super helix. It also indicated that the gene possesses a positive cluster and the negative pocket, suggesting a potential binding site for cell wall pectins and PG of pathogen.
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1 - Chung-Ang University, Dept. of Applied Plant Science, San 72-1, Naeri, Daeduckmyun, Anseong, Gyeonggido, 456-756, South Korea
2 - FnP Corp., Center for Fungi and Plant Genome Research
3 - National Horticultural Research Institute, Horticultural Biotechnology
4 - National Institute of Agricultural Biotechnology, Brassica Genomics Team
5 - Chung-Ang University, Dept. of Applied Plant Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM