| Abstract Detail
Symbioses: Plant, Animal, and Microbe Interactions Miller, Joseph T. [1], Hendrix, Stephen [2]. Plant genomic response to insect galling: The Solidago galling complex. The interactions of the genus Solidago, commonly known as goldenrod, with its insect guild represent one of the most well known plant-insect gall systems. We apply functional genomics techniques to discover and confirm differentially expressed plant genes in S. altissima plants galled by Eurosta to identify candidate genes for gall development. We generated tissue-specific cDNA libraries of galled and ungalled tissue and reciprocally subtracted them to generate cDNAs specific to galled and ungalled tissue. These libraries are them spotted on mircorarray slides. The resultant microarrays were hybridized with multiple replicates of RNA extracted from pairs of galled and ungalled Solidago stems. ESTs determined to be differentially expressed in the microarray study were sequenced and a subset confirmed by real-time PCR. Our results have identified differentially expressed genes involved in multiple growth regulating modes: abscisic acid, gibberellic acid, jasmonic acid, salicylic acid, polyamines and ethylene. In particular several gene regulated by abscisic acid are up-regulated in the galled tissue. These data are helping us develop hypotheses that hormones are regulating cell growth in the pith tissue, which is the site of larval growth. Future studies will test the candidate gene expression levels in S. altissima galls formed by the moth Gnorimoschema gallaesolidaginis. Since these two insects also form galls on a closely related Solidago species, S. gigantea the comparative approach can be used to test the gene expression patterns on the second plant species. Temporally conserved expression patterns among genotypes and gallers will provide evidence of their role in gall development specific to a galler or conserved among phylogenetically differentiated galling insects.
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Related Links:
http://ccg.biology.uiowa.edu/jmiller/solidago.php
1 - University of Iowa, Biology Department, 138 Biology Building, Iowa City, Iowa, 52242, USA
2 - University of Iowa, Department of Biology, 143 Biology Building, Iowa City, IA, 52242, USA
Keywords:
none specified
Presentation Type: Oral Paper:Papers for Topics
Session: CP51
Location: Continental B/Hilton
Date: Wednesday, July 11th, 2007
Time: 2:00 PM
Number: CP51005
Abstract ID:1498 |
