Wu, Hui-Chen , Jinn, Tsung-Luo .
A Versatile Calmodulin is Temperature-dependent Sensor.
The calcium/calmodulin (CaM) transduction pathways participated in early heat shock (HS) response and thermotolerance in rice (Oryza sativa L.) seedlings was investigated. HS initiates Ca2+ signal which is spatially distinct and the heat-stress-increased Ca2+ transients mainly from extracellular source are correlated with CaMs and OsHSP genes expression is confirmed. A significant increase of [Ca2+] i was observed during early HS response at 37C using Fluo-3/acetoxymethyl esters and laser scanning confocal microscopy. Meanwhile exogenous application of Ca2+and A23187 also accelerate OsCaM1 and OsHSP genes expression. For turn on OsHSP genes in response to HS is depended on Ca2+ oscillations, up-down, up-down, which may interpret by OsCaM1 is proposed. OsCaM1-GFP mobilizes from extracellular to cytosol within 10 to 16 min with temperature-dependent manner. When over expression of OsCaM1 in Arabidopsis accelerate HSP genes expression during early HS response. These results provide direct evidence that Ca2+/calmodulin mediated HS signaling is critical for thermotolerant acquisition in planta.
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1 - National Taiwan University, Department of Life Science and Institute of Plant Biology, R913 Life Sci Bldg, NTU, Taipei, 10617, Taiwan
2 - National Taiwan University, Department of Life Science and Institute of Plant Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM