Mechanisms of Gene Regulation
Johnson, Eric T. , Dowd, Patrick F. .
Excision of the piggyBac transposable element in maize cells is a precise event.
The piggyBac transposable element (TE) from the moth Trichoplusia ni encodes a ‘cut and paste’ DNA transposase that has been used to transform a number of insects, as well as planaria, mammalian cells and mice. The wild type and a mutated piggyBac TE excised from a DNA vector in transient assays using maize cells when the piggyBac transposase was simultaneously expressed by a maize ubiquitin promoter. The mutated piggyBac TE also excised in the presence of the piggyBac transposase in stably transformed maize cells, leaving behind a ‘TTAA’ DNA sequence in its place, indicating a precise molecular excision, characteristic of piggyBac transposase in other eukaryotic cells. These data indicate that piggyBac transposons may be able to carry genes into maize cells or serve as a mutagen for genetic studies.
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1 - USDA-ARS, Crop Bioprotection Research Unit, 1815 N. University St., Peoria, IL, 61604, USA
2 - USDA-ARS, Crop Bioprotection Research Unit
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM