Protein Targeting and Vesicular Trafficking
Jou, YingTzy , Chen, Yu-Chan , Yen, Hungchen Emilie .
Salinity-induced changes in cellular localizations of SKD1 and two SKD1-interacting proteins SNF1 and CPN1 in Mesembryanthemum crystallinum.
A salt-induced protein mcSKD1 (suppressor of K+ growth defect) is involved in the intracellular vesicular trafficking in halophyte ice plant (Mesembryanthemum crystallinum L.). Two proteins interacted with mcSKD1, namely mcSNF1 and mcCPN1, were isolated by yeast two-hybrid screen and the interactions were confirmed by immunoprecipitation. Sequence analysis showed that mcSNF1 and mcCPN1 contain an ubiquitin-binding domain and RING domain, respectively, suggesting both are involved in ubiquitination processes. The distributions of mcSKD1, mcSNF1, and mcCPN1 in the microsomal fractions were altered by 200 mM NaCl salt stress. Salinity increased the fractions of mcSKD1 interacted with mcSNF1. Immunocytochemistrical observations showed that mcSDK1 formed small aggregates associated with ER-Golgi network in the cytosol, and redistributed to the plasma membrane and central vacuole within 1 h of salt-stressed. The mcSNF1 and mcCPN1 also showed similar patterns of redistribution at early stage of salt stress. After 6 h of salt stress, most of the mcSKD1 and mcSNF1 proteins were found in the plasma membrane, while mcCPN1 was found to associate with ER-Golgi network. The rapid shuttling of these three proteins between membrane compartments in relation to salt-tolerant mechanism of ice plant will be discussed.
Log in to add this item to your schedule
1 - National Chung-Hsing University, Department of Life Sciences, 250, Kuo Kuang Rd.,, Taichung, Taiwan
2 - National Chung-Hsing University, Department of Life Sciences
3 - National Chung-Hsing University, Department of Life Sciences
plant cell biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM