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Abstract Detail


Emerging Technologies

Nakanishi, Tomoko [1], Tanoi, Keitaro [2], Ohya, Tomoyuki [3].

Water specific imaging and measurement in a living plant.

Water plays an important role in plant activity. However, tools for water specific imaging and measurement in a living plant are limited. We have been studying water specific imaging and measurement using neutron beam and radioisotopes, respectively. Through neutrons, we could obtain water profile in a living plant as well as in soil around the root. The obtainable resolution of 2-dimensional image and spatial one is now about 20 μm and 100 μm, respectively. With neutron imaging method, water in the vicinity of the soybean root, imbedded in soil was analyzed nondestructively. To study water movement, we employed positron emitters to label water. Applying tritium, 3H, is one of the candidate to label water. However, beta-ray energy of 3H is too low to detect from outside of the plant. Therefore, first of all, we produced 18F, a positron emitting nuclide, through irradiation of water by helium beam. Using 18F to trace water movement, we could study water absorption manner by a cowpea plant under water deficient condition. However, positron is liable to escape from thin tissue like leaves, therefore, it was not able to determine water amount moving in a plant. To determine water amount moving in a plant, we developed the system to measure the amount of water at 1cm stem. Then we produced 15O-labeled water and applied to a soybean plant from roots. It was shown that tremendous amount of water was leaking out from xylem cells and re-entered to the cells in an internode between cotyledon and the first leaf. Since the half-life of 15O is only 2 minutes, we could perform the experiment for about 1000 seconds. Through simulation, about half of all the water existed in 1cm stem was replaced with newly absorbed water within about 20 minutes.


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1 - University of Tokyo, Graduate School of Agricultural and Life Sciences, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
2 - Univedrsity of Tokyo, Graduate School of Agricultural and Life Sciences
3 - University of Tokyo, Graduate School of Agricultural and Life Sciences

Keywords:
water imaging
water transport
nondestructive measurement
bean
neutron beam
positron emitter
18F
15O.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44016
Abstract ID:1435


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