| Abstract Detail
Plant biotech & Risk Assessment Borisjuk, Nikolai [1], Hensel, G. [2], Kumlehn, Jochen [3], Golovkin, Maxim [4], Spitsin, Sergei [4], Andrianov, Vyacheslav [4], Pogrebnyak, Natalia [4], Smirnov, Yuri [4], Brodzik, Robert [4], Matyszczuk, Paulina [4], Koprowski, Hilary [4]. Expression of avian flu antigen for bird immunization. Our strategy to generate a vaccine against the H5N1 influenza virus relies on the expression of virus surface antigen hemagglutinin (HA) in plant material that could be used as poultry feed and to use this feed for massive oral bird immunization. We tested the following expression systems: stably transformed tobacco and barley, and a transient plant virus expression system, MagnICON (Icon Genetics, Germany). Following tobacco transformation with hemagglutinin gene variants, driven by two strong promoters, we were not able to detect any HA expression. Using MagnICON, we succeeded in expressing HA1 at a well detectable but relatively low protein level. The truncated HA1 polypeptide lacking the trans-membrane domain has been expressed in tobacco, N. benthamiana and Swiss chard (Beta vulgaris var. cycla) plants, at the level sufficient for immunological testing. The best expression of HA1 antigen has been achieved in mature barley seeds under control of the seed-specific α-gliadin promoter of wheat. A particularly high expression of codon-optimized HA1 was detected by Western blot analysis in seeds of two out of 84 transgenic barley lines generated. The immunological evaluations of plant-expressed avian flu hemagglutinin antigen variants will be discussed.
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1 - Thomas Jefferson University, 1020 Locust Str., Philadelphia, PA, 19107, USA
2 - Institute of Plant Genetics, Gatersleben, Germany
3 - Institute of Plant Genetics, Gatersleben, Germany
4 - Thomas Jefferson University
Keywords:
avian flu
barley seeds
antigen
transient expression.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P45020
Abstract ID:1410 |
