Duval, Isabelle , Moneva, Evguenia , Beaudoin, Nathalie .
Transcriptional profiling in response to inhibition of cellulose synthesis.
Thaxtomin A is the main phytotoxin produced by Streptomyces scabiei, an important causal agent of common scab disease in potato. Thaxtomin A has been shown to inhibit cellulose synthesis in Arabidopsis thaliana seedlings. Moreover, we have shown that thaxtomin A activates in arabidopsis cell suspensions a program of cell death associated with DNA fragmentation and dependant on de novo gene transcription and protein synthesis. However, this form of programmed cell death (PCD) is different from the so called hypersensitive-cell death since addition of thaxtomin A does not induce the expression of typical defence-associated genes. Interestingly, treatment of cell suspensions with another inhibitor of cellulose synthesis, isoxaben, also activates a similar program of cell death in cell suspensions. These results suggest that inhibition of cellulose synthesis leads to the activation of PCD. In order to identify genes whose product may be implicated in the induction of this type of PCD, micro-array analyses have been performed on arabidopsis cell suspensions treated with thaxtomin A or isoxaben. Our results indicate that close to 75% of the genes encoding known proteins are induced similarly by both treatments. Comparison with other micro-array analyses suggests that inhibition of cellulose synthesis activates wound-like responses in arabidopsis cell suspensions.
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1 - Universite de Sherbrooke, Biology, 2500 Boul. Universite, Sherbrooke, Quebec, J1K 2R1, Canada
Programmed Cell Death
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM