Cytoskeleton Structure and Dynamics
Maximova, Eugenia .
Colocalization of intracellular filaments with the vacuoles in carpocallus cells.
Over the past several years and decades most often was demonstrated that intracellular space represents a high organised and orchestrated complex of organelles and suborganelles involved in movement and trafficking of primary and secondary metabolites, cell division, cell polarity and others vital processes.These spatio-temporal processes are accompanied by different phenomena and modes of assembly or disassembly, among them: adhesion, coherence, congruence, anchorage, co-couplation, colocalization, branching, disrupting, exclusion, etc. The phenomenon of colocalization of filamentous elements with tonoplast membrane was short noticed in our publications. Indeed, the analysis of electron micrographs of fine sections from callus of red cell line demonstrated the cyanogenic particle-filament-anchoring assemblies and high organised parallel arrays along the surface plan of vacuoles. The perivacuolar filaments curved and turned to the “gate-pore” of tonoplast, where dipped the electron-dense material of phenolic compounds into vacuolar lumen. Therefore, the complex of filament-tonoplast colocalization with electron-dense cargoes of large particles represents not a simple collateral adhesion, but a multipartite strong vectorised functional assembly which provided the translocation and storing of secondary metabolites. Displacement process comprises the motor (myosin), rail (actin) and associated proteins. Consistent with the opinion of Hill et.al. (1996) the colocalization of actin with the vacuole membrane corroborates the genetic evidence, suggesting that actin filaments are directly involved in vacuole inheritance. In our opinion the colocalization reflects an evolutionary advent in cell competence development of mutual events.
Log in to add this item to your schedule
1 - MPI of Molecular Plant Physiology, Microscopy Unit, am Mühlenberg, 1, Golm, 14476, Germany
colocalization of filaments.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM