Ibraheem, Farag , Chopra, Surinder .
The sorghum transcription factor yellow seed 1 (y1) regulates biosynthesis of defense related compounds.
Phytoalexins are low molecular weight antimicrobial compounds produced in response to biotic and abiotic stresses. Engineering of these compounds in economically important crops has emerged as a valuable approach for enhancing resistance of these crops against pathogens. Sorghum plants respond to pathogenic (Colletotrichum sublineolum) and non pathogenic (Cochliobolus heterostrophus) fungi by production of phytoalexins of the 3-deoxyanthocyanidin flavonoid type. These compounds accumulate in leaf and mesocotyls at the site of infection. They have been identified as luteolinidin, apigeninidin, and their derivatives. The biosynthetic pathway that synthesizes these compounds is not well characterized. 3-deoxyanthocyanidin phytoalexins have chemical structure similarity to the precursors of the red phlobaphene pigments that accumulate in sorghum and maize seed pericarp. We have cloned a sorghum myb transcription factor yellow seed1 (y1) which controls pholbaphene biosynthesis. y1 is expressed in leaf tissues and is induced by fungal infection. We have used tansposon-mediated mutagenesis to generate sorghum lines with mutations in y1 locus. In the current work, we have used these lines to understand biosynthesis of defense related compounds. Further, the heterologus expression of sorghum y1 is being characterized in maize and results indicate that y1 is active in maize tissues. Results describing the constitutive and induced expression of y1 in maize will be displayed.
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1 - The Pennsylvania state university, 1 Crop and soil sciences, 2 Intercollege graduate degree program in plant biology, University Park, State College, PA, 16802, USA
2 - The Pennsylvanis state university, 1 Crop and soil sciences, 2 Intercollege graduate degree program in plant biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM