Zhang, Ji-Yi , Wang, Zeng-Yu , Schachtman, Daniel P. , Udvardi, Michael K. .
Improving Drought Tolerance in Medicago truncatula via translational genomics.
Drought is one of the most important barriers to agricultural expansion, leaving regions of the globe uncultivated or with low crop yield. Drought negatively effects many legume crops, including soybean, alfalfa, clovers, pea, and beans. A translational genomics program has been initiated to improve drought tolerance in legumes. The model legume Medicago truncatula is being used to assess the effects of various transgenes on abiotic stress tolerance in this species. Among the genes chosen for drought tolerance are four encoding transcription factors (AP2/ERF-like MtWXP1 and 2, and MtNAC1 and 2), MtNCED1 encoding the NCED enzyme that participates in ABA biosynthesis, a proton-pump gene AtAVP1, and the protein kinase gene AtSnRK2.8. Transgenic plants are being created using cotyledon explants, followed by regeneration via direct organogenesis. At the same time, M. truncatula is being used as a model plant to build a legume-specific regulatory gene network responding to dehydration stresses, in order to understand the drought adaptation of legumes, and to identify novel genes involved in drought and other abiotic stress tolerance. The Medicago Gene Atlas based on the Affymetrix platform is a complementary tool in this program, allowing the evaluation of the spatial transcriptional profile of genes. It will be broadened by adding transcriptome with a planned new series of chips under drought and other abiotic stress conditions.
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1 - The Samuel Roberts Noble Foundation, Plant Biology Division, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA
2 - The Samuel Roberts Noble Foundation, Forage Improvment Division, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA
3 - Donald Danforth Plant Science Center, 975 N. Warson Rd, St. Louis, MO, 63132, USA
4 - The Samuel Roberts Noble Foundation, Inc., Plant Biology Division, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM