Wu, Yujia , Maxon, Susan , Payne, Richard .
Improved Method for Wheat Varietal Identification by Double Staining IEF Polyacrylamide Gel.
Wheat (Triticum aestivum L.) is one of the main cereal crops in worldwide production today. It is not only an important crop for human consumption and animal feed, but is also a unique crop for industrial uses and for various other products. Since numerous varieties are cultivated for many purposes around the world, wheat variety tests can be very important in identifying contaminated seed and mislabeled seed lots. In conjunction with anatomical differences in wheat seed, protein electrophoresis is a powerful method of identification of wheat varieties from seeds. A new procedure using IEF techniques has been developed, based on seed protein characteristics and isoelectric points. Using a small vertical, polyacrylamide plate, IEF gel system with Coomassie blue and esterase double staining reduces time and therefore cost. The IEF gel system is well suited for wheat seed protein analysis, and comparisons of SDS denatured PAGE with IEF native gels show IEF is a good alternative. When numerous varieties were tested, esterase staining (brown color) generated several banding patterns; the addition of a second total protein staining using Coomassie blue (blue color) created an enhanced new banding pattern, increasing the power of the test to differentiate varieties. The IEF gel system plus double-staining produces high-resolution bands that may be different than, or in addition to, those visible with SDS-PAGE. This system employs a simple extraction and fast set-up, is lower cost, and generates highly resolved bands, making it easy to differentiate between varieties of cultivated wheat seed.
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1 - USDA-AMS, Seed Regulatory and Testing Branch, Federal Seed Laboratory, 801 Summit Crossing Place, Suite C, Gastinia, North Carolina, 28054, USA
2 - USDA-AMS, Seed Regulatory and Testing Branch, Federal Seed Laboratory
wheat varietal identification
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM