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Abstract Detail


Environmental Physiology

Si, Ying [1], Dane, Fenny [2].

Drought induced gene expression in Citrullus colocynthis.

Citrullus colocynthis (L.) Schrad, closely related to watermelon, is a member of the Cucurbitaceae family. This plant, commonly known as the bitter apple or bitter gourd, is a very drought tolerant species with deep root system, widely distributed in the Sahara-Arabian deserts in Africa and the Mediterranean region. cDNA-AFLP was used to study differential gene expression in roots following a 20% polyethylene glycol (PEG8000) induced drought stress treatment. Twenty genes which show similarity to known function genes were confirmed by relative real-time RT-PCR to be up-regulated. Real-time RT-PCR was also used to quantify the expression of these genes originating from roots in root and shoot tissues at five time points (4, 8, 12, 24, and 48 hr respectively). In the most of cases, the highest induction levels were detected in roots between 4 hr and 12 hr of drought stress, whereas in shoots between 12 hr and 48 hr. Some genes significantly up-regulated in roots or in shoots, were only slightly up-regulated in shoots or in roots, respectively. Seedlings were treated with three plant hormones, salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA) individually and analyzed using real-time RT-PCR. A complex interplay between ABA, JA and SA signaling pathways regulate plant gene expression during adaptive responses to abiotic stress.


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1 - Auburn University, Horticulture, Funchess Hall 101, Auburn, AL, 36849, USA
2 - Auburn University, Horticulture

Keywords:
drought stress
cDNA-AFLP
gene expression
Citrullus colocynthis
real-time RT-PCR.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P01027
Abstract ID:1362


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