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Abstract Detail


Plant-Pathogen Interactions

Reinprecht, Yarmilla [1], Wu, Xiaoguang [2], Luk-Labey, Shun-Yan [2], Martin, Christopher J. [2], Pauls, K. Peter [2].

Fusarium graminearum resistance in corn (Zea mays L.).

Fusarium graminearum causes gibberella ear rot in corn. The incidence and severity of the disease is strongly influenced by environmental factors and infection can result in severe reduction in crop quality because it contaminates the grain with trichothecene mycotoxins which are dangerous to humans and animals. The most effective method of control would be to use resistant hybrids but most commercial corn hybrids have little or no resistance to this pathogen. The objectives of this work were to define the genes underlying QTL previously identified for resistance to giberella ear rot in corn and to develop gene-based markers for resistance to infection by F. graminearum. Approximately 300 sequence-specific PCR primers were designed (based on ESTs for Fusarium infected corn, markers associated with FHB in wheat and barley, ESTs that have been mapped to the Fusarium QTL regions in other studies and likely candidate genes such as ferulic acid synthesis, RPL3 and G proteins) and screened with the parents of the mapping population (CO387, resistant and CG62, susceptible). Polymorphic markers were mapped and monomorphic fragments were cloned and sequenced to produce SNP markers. To date, 100 polymorphic markers have been scored in the mapping population. 71 markers have been used in JoinMap analysis. Some of the new markers cosegregate with previous Fusarium resistance QTL. The identified resistance genes will be converted to easily scored markers to allow rapid introgression of gibberella ear rot resistance into elite germplasm.


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1 - University of Guelph, Plant Agriculture, Crop Science Bldg, Guelph, Ontario, N1G 2W1, Canada
2 - University of Guelph, Plant Agriculture

Keywords:
Corn
Fusarium graminearum
QTL
resistance genes
gene-based markers.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15051
Abstract ID:1355


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