Unable to connect to database - 08:13:24 Unable to connect to database - 08:13:24 SQL Statement is null or not a SELECT - 08:13:24 SQL Statement is null or not a DELETE - 08:13:24 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 08:13:24 Unable to connect to database - 08:13:24 SQL Statement is null or not a SELECT - 08:13:24

Abstract Detail


Secondary Metabolism

zhou, lili [1], zeng, hainian [1], shi, mingzhu [1], xie, deyu [1].

Establishment of transgenic anthocyanin-producing tissue culture over-expressing a R2R3-MYB trascriptional factor.

Anthocyanins are one group of the richest plant pigments playing numerous significant roles essentially involved in plant reproduction and protection. The biosynthesis and regulation of anthocyanins have been intensively studied in plants, including the cloning and characterization of all pathway and many regulatory genes. However, many questions in regarding to the regulation of the biosynthesis of anthocyanins remain open. We report here the establishment of anthocyanin-producing tissue culture and cell suspension from a purple colored transgenic tobacco plant constitutively expressing an Arabidopsis PAP1 gene, which encodes a R2R3-MYB transcriptional factor essentially involved in the regulation of anthocyanins biosynthesis; and the regulation of anthocyanin formation. Using callus induction media consisting of MS medium containing different combinations of plant growth regulators of NAA/2,4-D and kinetin, supplemented with antibiotic kanamycin, friable red cell lines (consistently accumulating red pigment) and white cell lines (hardly accumulating pigment) were differentially developed from the same leaf tissues of the transgenic plant. BY-cells from leaf tissues of wild type tobacco plants were obtained as control on the same induction medium excluding kanamycin. The effects of plant growth regulators on cell growth and anthocyanin formation were conducted. The combinations of 0.1-0.3 mg/L NAA/0.11 mg/L kinetin promoted the growth of transgenic purple cells and the production of anthocyanin. HPLC-MS based profiling showed the red cells produced at least 5 different anthocyanin molecules. More details of cell growth, anthocyanin production, and the effects of different factors on the regulation of anthocyanin biosynthesis and anthocyanin structures will be presented and discussed in our presentation.


Log in to add this item to your schedule

1 - North Carolina State University, Plant Biology, 2115 Gardner Hall, Campus Box 7612, Raleigh, NC, 27695, USA

Keywords:
PAP 1
transgenic
transcription factor
R2R3-MYB
HPLC.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20035
Abstract ID:1336


Copyright © 2000-2007, Botanical Society of America. All rights