Yoo, Cheol Min , Blancaflor, Elison B .
AtCSLD2 is another cellulose synthase-like gene important for root hair development in Arabidopsis.
We identified an Arabidopsis mutant with altered root hair morphology from a screen of T–DNA mutagenized seedlings. The mutant root hairs showed swelling along the base and a leaky cytoplasm, which reduced the root hair length substantially. The phenotype of this mutant was reminiscent of the phenotype of the AtCSLD3 mutants, kojak (kjk-1) and csld3-1(Favery et al. 2001; Wang et al. 2001). We determined that this mutant had a T-DNA insertion at the first exon of the gene that encodes AtCSLD2 (At5g16910), another cellulose synthase-like gene, which has 88% identity to the amino acid sequence of AtCSLD3. We also obtained a homozygous mutant in the AtCLSD3 gene from the ABRC with a T-DNA insertion at the first exon. The mutant root hairs exploded immediately after initiation similar to the kjk-2 mutant. When we crossed the clsd2 with the csld3 mutant, F1 seedlings showed a similar root hair phenotype as the homozygous csld2 mutants. Furthermore, the F1 plants produced aborted seeds in their siliques indicating that clsd2/clsd3 double knockouts are lethal. Interestingly, when the csld2 and clsd3 mutant seedlings were grown at 15 degree C, the root hairs elongated to a greater extent than the seedlings grown at 25 degree C. In addition, the root hair cytoplasm of clsd2 mutants was less leaky and root hairs of csld3 mutants produced branches when grown at low temperature. In vivo labeling of F-actin and microtubules using GFP fusions revealed disruption in the root hair cytoskeleton of both mutants. Taken together, our results demonstrate that AtCSLD2 is another cellulose synthase-like gene that is important for Arabidopsis root hair development possibly through the regulation of cell wall synthesis at the growing root hair tip.
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1 - Samuel Roberts Noble Foundation, Plant Biology Division, 2510 Sam Noble Parkway, Ardmore, Oklahoma, 73401, USA
2 - Samuel Roberts Noble Foundation, Plant Biology Division
cellulose synthase like gene.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM