Salomé, Patrice A. , Weigel, Detlef .
Cryptic variation in circadian function and flowering time in Arabidopsis.
The daily synchronization of the internal circadian clock to the outside environment is crucial for most organisms. An important use of the circadian clock is the adjustment of flowering to seasonal changes in day length. Most strains of Arabidopsis flower in spring or early summer, once the critical daylength has passed a certain threshold. Day length perception relies on the timing of expression of CONSTANS, which induces the flowering activator FT. There is, however, great variation in the photoperiodic flowering behavior of Arabidopsis strains, some of which is likely due to variation in or downstream of the clock.
We are investigating variation in clock function and flowering behavior using a combination of approaches: 1) Assaying a TOC1:LUC reporter and performing whole-genome expression profiling of wild Arabidopsis strains (accessions). 2) Identification of natural modifiers of TOC1:LUC expression in progeny from crosses between Col-2 and chosen accessions. We are focusing on crosses in which the variation can be fully explained by the contribution of only one or two genes. 3) Inducing artificial miRNA-directed loss of clock gene function in 19 accessions. We are manipulating the activity of clock genes in these accessions, with the goal of recording circadian parameters and flowering time in the lines generated. This will allow us to measure background dependent gene activity for several critical components of the clock. Our combined approach will enable both a correlation analysis between circadian and flowering phenotypes, and identification of genes governing these differences.
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1 - Max Planck Institute for Developmental Biology, Abteilung 6, 37-39 Spemannstrasse, Tuebingen, 72076, Germany
2 - Max Planck Institute for Developmental Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM