Nishiyama, Tomo , Choi, Sang Ja , Shinano, Takuro , Koyama, Hiroyuki , Ito, Susumu , Wasaki, Jun , Osaki, Mitsuru .
Analysis of OsPI1, Rice Non-coding RNA Responsible for Phosphate Deprivation.
For the adaptation on P deprivation, plant have developed several strategies, which are able to classify into 1) positive P acquisition from rhizosphere and 2) efficient P recycling in plants. OsPI1 (Oryza sativa phosphate-limitation inducible gene 1) was isolated from rice by microarray analysis as one of the genes responding to P deprivation. It has been revealed that OsPI1 codes a non-coding RNA and immediately responses when the plant faces to P deprivation. OsPI1 is suggested to belong to the TPSI1/Mt4 family, which is a P deprivation inducible non-coding RNA family. And we assumed that OsPI1 has a function of P recycling, as well as At4, one of the orthologs isolated from Arabidopsis. The aim of this study is to investigate the function of OsPI1 in adaptation on P deprivation. Two OsPI1 knockdown lines were constructed by RNAi. Growth of knockdown lines under P deprivation decreased in comparison with wild type, while P concentration of knockdown lines was still higher. This result indicates that OsPI1 expression regulates P recycling system. To confirm this idea, microarray analysis was conducted to knockdown line and wild type which are grown under P sufficient and deficient conditions. Transcriptomic analysis revealed that the increase of mRNA for several genes involved in P translocation was repressed in knockdown line. Then, split roots of rice seedlings were transferred into nutrient solution with or without P and used for analysis of OsPI1 expression. mRNA for OsPI1 was disappeared immediately when P was re-supplied. This result suggests that OsPI1 expression is controlled depend on P condition around plant root. In conclusion, it was shown that OsPI1 had an important role in P recycling in plant.
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1 - Hokkaido Univ., Graduate School of Agriculture
2 - Hokkaido Univ., CRIS
3 - Gifu Univ., Faculty of Applied Biological Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM