| Abstract Detail
Plant biotech & Risk Assessment Jenkin, Jody [1], Dang, Benyuan [2], Gintz, Rebecca [2], Tsai, Fei-Yi [3], Chen, Zhizheng [2]. Transformation Systems of Canola Using Anthranilate Syntheses Gene ASA2 as a Selectable Marker. Recently, a number of new selectable marker genes have been reported for plant transformation technologies. Dr.J.M.Widholm’s Lab isolated the feedback-insensitive anthranilate synthase cDNA (ASA2) from a 5-methyltryptophan (5-MT)-resistant tobacco cell line and investigated it as a selectable marker for the transformation of the legume A.sinicus. Most of the analogs of tryptophan, indole and anthranilate are shown toxic to the plants and inhibit tryptophan synthesis since the analogs bind to anthranilate synthase. The ASA2 gene can detoxify the analogs since it was isolated from 5-MT resistant cell lines. ASA2 gene naturally occurs in tryptophan biosynthesis; therefore it should have no detrimental effects if transferred into other plants or microbes.
In this study, we reported ASA2 gene was tested in Agrobacterium tumefaciens-mediated and biolistic transformation systems on Canola and found it may be used as a selectable marker in biolistic transformation system, but not in A. tumefaciens-mediated transformation system on Canola.
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1 - Cargill Inc., Cell Biology, 2540 East Drake Road, Ft. Collins, CO, 80525, US
2 - Cargill Inc., Cell Biology
3 - University of Illinois, Department of Crop Science
Keywords:
Canola.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P45018
Abstract ID:1294 |
