| Abstract Detail
Membrane Transport Vincill, Eric [1], Roberts, Daniel [2]. Nodulin-Like Transporters From Arabidopsis Are Induced by Wounding and Salicylic Acid. GmN70 is a 12-transmembrane inorganic anion transporter belonging to the multi-gene Nodulin-Like Anion Transporter (NLAT) family of proteins which is expressed on the symbiosome membrane of Soybean nodules. Arabidopsis has homologs of GmN70, suggesting that NLAT’s have other functions in plants besides a symbiotic role. In this study we characterize two homologs of GmN70 in Arabidopsis. AtNLAT1 and AtNLAT2 have a high sequence identity (respective 63.7% and 56.3%) and similar protein topology as GmN70. By using a combination of Q-PCR and promoter-GUS reporter transgenic lines, the expression pattern of AtNLAT1 and AtNLAT2 was investigated. Both AtNLAT1 and AtNLAT2 are up-regulated by wounding and senescence with NLAT promoter-GUS transgenic lines showing up-regulation in leaf vasculature. Treatment with the wound response hormone jasmonic acid had no affect on transcript expression of either AtNLAT1 or AtNLAT2. Treatment with the defense hormone, salicylic acid (SA, 5 mM), induces a 7-8 fold increase in AtNLAT1 expression in leaves. Further investigation with the AtNLAT1 promoter-GUS reporter transgenic line identified specific expression in the trichome upon SA challenge. Over-expression transgenic lines of AtNLAT1 in Arabidopsis showed a reduced trichome number and altered leaf morphology. Overall, the data suggests that AtNLAT1 and AtNLAT2 may be important in plant defense responses and senescence. (Supported by NSF grant MCB-0237219)
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1 - University of Tennessee, Biochemistry and Molecular Biology, F431 Walters Life Science, Knoxville, TN, 37996, United States
2 - University of Tennessee, Biochemistry and Molecular Biology
Keywords:
salicylic acid
defense
senescence
wounding.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P10009
Abstract ID:1292 |
