Growth and Vegetative Development
Dubrovsky, Joseph , Hernández-Barrera, Alejandra , Lira-Ruan, Verónica , Shishkova, Svetlana , Soukup, Aleš , Reyes-Hernández, B. Jazmin , Dong, Gaofeng .
Arabidospsis mutants with determinate primary root and alterations in root architecture.
Root meristem maintenance is one of the key topics of plant development. Several mutants altered in root development and root system architecture were isolated after screening of more than 17,000 M2 plants of EMS-mutagenized Arabidopsis thaliana. The mutants preliminarily called sr (“short roots with determinate growth”) and dg (“determinate growth”) have alterations in primary root growth. Both mutants showed disorganization in the root apical meristem occurring within the first week of growth. The primary root mersitem became completely exhausted between 13 and 16 days after germination in these mutants. Root architecture was abnormal in both mutants. Many short pericycle cells adjacent to the protoxylem were found in sr, indicating alterations in lateral root initiation. The mutant forms many short lateral roots. The morphogenesis of the lateral root primordium in sr is also altered. In dg, apparently, lateral root development is unaltered. However, this mutant forms extra-numerous adventitious roots unusually spaced, the feature that alters significantly the root architecture. This mutant is delayed in many developmental aspects, including flowering time. Genetic analysis proved that both sr and dg are monogenic recessive mutations. Genetic mapping, using SSLP markers are in progress. For the moment, SR is mapped on the lower arm of chromosome 4 and DG gene is mapped on chromosome 5 between 23.7 y 29 cM. The data on cellular analysis of root development in these mutants will be presented. We thank S. Napsucialy-Mendivil for excellent technical help and DGAPA-PAPIIT, UNAM (projects IN 225906 and IN227206) for financial support.
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1 - Instituto De Biotecnología, Universidad Nacional Autónoma de México, Biología Molecular de Plantas, Apdo. Postal 510-3, Cuernavaca, México., Morelos, C.P. 62250, Mexico
2 - Instituto de Biotecnología, Universidad Nacional Autónoma de México, Biología Molecular de Plantas
3 - Instituto De Biotecnología, UNAM and Fac. Ciencias UAEM
determinate root growth.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM