| Abstract Detail
Membrane Transport Kuznetsov, Vladimir V. [1], Kulikova, Alevtina L. [2], Meshcheryakov, Anatolii B. [2], Smolenskaya, Irina N. [3], Kholodova, Valentina P. [3]. Participation of actin cytoskeleton in intracellular hexose transporter trafficking. Sugars of a nutritional medium are the sole source of organic matters for sugar beet cells grown as an isolated suspension culture. Until sugar concentration in medium was rather high, an activity of the high-affinity hexose membrane transport system was very low. The induction of 10 μM 3-O-methyl-D-glucose (MGl) membrane transport was strongly initiated when the plant cells were staying in medium without sugars (“starvation”) for a few hours. Effects of some drugs were compared to rates of a basic or an activated MGl transport into sugar beet suspension cells and to a state of their actin cytoskeleton (AC). Falloidin, cytochalasin B and D, latrunculin or brefeldin A which possess to modify AC did not significantly influence to rates of the basic MGl transport into sugar beet cells. No effect was shown for falloidin, AC stabilizing drug, to rates of activated MGl transport as well. But latrunculinB, cytochalasin B or D, disrupting actin microfilaments and reducing F-actin content in cell extracts, decreased the rate of activated MGl transport in dose-dependent manner. The highest concentration of latrunculin B used – 2.10-5 M – inhibited completely activation of MGl transport to the level of the rate of the basic, non-activated hexose uptake by the cells. Brefeldin A which suppressed intracellular vesicular trafficing, did not affect AC content but inhibited significantly the rate of MGl activated membrane transport. Since the activation of membrane transport was due to additional transporter assembles, its inhibition with the help of the AC modifiers used means the participation of AC in intracellular vesicular hexose transporter trafficking or their insertion into plasma membrane. The study was supported by the Program “Cell and Molecular Biology”, Presidium, RAS.
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vesicular traffick
1 - Institute of Plant Physiology RAS, Laboratory of Plant Adaptation to Extreme Environment, Botanicheskaya ul. 35, Moscow, 127276, Russia
2 - Institute of Plant Physiology RAS
3 - Institute of Plant Physiology RAS, Botanicheskaya ul. 35, Moscow, 127276, Russia
Keywords:
actin and hexose transporter.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P10008
Abstract ID:1228 |
