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Abstract Detail


Metabolism

Orcaray, Luis [1], Igal, Maria [2], Zabalza, Ana [2], Royuela, Mercedes [3].

Induction of ethanolic fermentation by the herbicide glyphosate.

Previous studies have shown that herbicides inhibiting the biosynthesis of branched-chain amino acids elicit an induction of aerobic fermentation. (Gaston et al., 2002). The aim of this work was to evaluate if fermentative induction is a general physiological effect of herbicides inhibiting the amino-acid biosynthesis and not only a specific efffect of branched-chain amino acid biosynthesis inhibitors. In order to asses this, fermentative metabolism was evaluated in plants treated with glyphosate, herbicide that inhibits the biosynthesis of aromatic amino acids.
Pea plants (Pisum sativum L. cv. Snap Sugar Boys) were grown in hydroponic tanks continously aerated. 2.35 mM glyphosate was applied to the nutrient solution when plants were 12-days old. Ethanolic fermentative enzymes, Pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH), were evaluated spectrophotometrically and by western-blotting 1,3 and 7 days after the onset of the treatment.
Specific activities of PDC and ADH were significantly increased after glyphosate treatment, within 3 and 7 days respectively. Western-blotting showed that both activities enhancement after glyphosate treatment was due to an induction of proteins expression. This fermentative induction after treatment with glyphosate (inhibitor of aromatic amino acid biosynthesis) was later than fermentative induction after treatment with herbicides that inhibit branched-chain amino acid biosynthesis (sulfonylureas and imidazolinones) (Zabalza et al., 2005). These results suggest that fermentative induction might be a general physiological effect of herbicides inhibiting amino acid biosynthesis, probably mediated by an imbalance of free amino acid pools.
Gaston et al. (2002). Physiol. Planta. 114: 524-532.Zabalza et al. (2005) J. Agric. Food. Chem. 53:7486-7493.


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1 - Universidad Pública de Navarra (Pamplona, Spain), Ciencias del Medio Natural
2 - Universidad Pública de Navarra (Pamplona, Spain), Ciencias del Medio Natural
3 - Universidad Pública de Navarra, Ciencias del Medio Natural, Campus Arrosadía, Pamplona, E31006, Spain

Keywords:
herbicide
amino acid biosynthesis
fermentative metabolism.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P19029
Abstract ID:1221


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