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Abstract Detail


Growth and Vegetative Development

Lee, Hyoshin [1], Lee, Jae-Soon [2], Bae, Eun-kyung [1], Park, So-Young [1], Sjodin, Andreas [3], Noh, Eun-Woon [1], Jansson, Stefan [3].

Growth-phase-dependent gene expression of poplar cells.

Complex sequences of morphological and biochemical changes occur during the developmental course of a batch plant cell culture. However, little information is available about the changes in gene expression that could explain these changes, due to the difficulties involved in isolating specific cellular events or developmental phases in the overlapping phases of cell growth. In an attempt to obtain such information we have examined the global growth phase-dependent gene expression of poplar cells in suspension cultures by cDNA microarray analysis. Our results reveal that significant changes occur in the expression of genes with functions related to protein synthesis, cell cycling, hormonal responses and cell wall biosynthesis, as cultures progress from initiation to senescence, that are highly correlated with observed developmental and physiological changes in the cells. Genes encoding protein kinases, calmodulin and proteins involved in both ascorbate metabolism and water-limited stress responses also showed strong stage-specific expression patterns. Our report provides fundamental information on molecular mechanisms that control cellular changes throughout the developmental course of poplar cell cultures.


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1 - Korea Forest Research Institute, Biotechnology Division
2 - Korea Forest Research Institute, Biotechnology Division, Omokchundong 23, Suwon, 441-350, Republic of Korea
3 - Umea University, Department of Plant Physiology

Keywords:
DNA microarray
cell growth
gene expression
Populus
suspension culture.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26040
Abstract ID:1214


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