| Abstract Detail
Water Relations Mohammadi, Mohsen [1], Kav, Nat N. V. [2], Deyholos, Michael K. [3]. Transcriptional profiling of hexaploid wheat (Triticum aestivum L.) roots identifies novel, dehydration-responsive genes. We used a long-oligonucleotide microarray to identify transcripts that increased or decreased in abundance in roots of dehydration-tolerant hexaploid bread wheat, in response to withholding of water. We observed that the major classes of dehydration-responsive genes (e.g. osmoprotectants, compatible solutes, proteases, glycosyltransferases/hydrolases, signal transducers components, ion transporters) were generally similar to those observed previously in other species and osmotic stresses. More specifically, we highlighted increases in transcript expression for specific genes including those putatively related to synthesis of asparagine, trehalose, oligopeptide transporters, metal-binding proteins, the GABA shunt, and transcription factors. Conversely, we noted a decrease in transcript abundance for diverse classes of glutathione and sulfur related enzymes, specific amino acids, as well as MATE-efflux carrier proteins. From these data, we identified a novel, dehydration-induced putative AP2/ERF transcription factor, which we predict to function as a transcriptional repressor. We also identified a dehydration-induced “little protein” (LitP; predicted mass: 8kDa) that is highly conserved across spermatophytes. Using qRT-PCR we compared the expression patterns of selected genes between two related wheat genotypes that differed in their susceptibility to dehydration, and confirmed that these novel genes were highly inducible by water limitation in both genotypes, although the magnitude of induction differed.
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1 - The University of Alberta, Dept. of Biological Sciences & Dept. of Agricultural Food and Nutritional Science, 410 Ag/For Center, University of Alberta, Edmonton, Alberta, Alberta, T6G 2P5, Canada
2 - The University of Alberta, Department of Agricultural, Food, and Nutritional Science
3 - The University of Alberta, Department of Biological Sciences
Keywords:
dehydration
microarray
wheat
genomics
crop
roots
Opata
Drought
osmotic
qRT-PCR.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P09001
Abstract ID:120 |
