Symbioses: Plant, Animal, and Microbe Interactions
Joneson, Suzanne , Dietrich, Fred , Lutzoni, Francois , Armaleo, Daniele .
Differentially regulated algal genes in the lichen symbiosis.
Lichens are the perennial symbiotic association of green algae and/or cyanobacteria with filamentous fungi. We know nothing of how these symbionts find or recognize each other, or how they maintain this long-term relationship. Here we present the first investigations into differentially expressed algal genes in early lichen development including pre-contact, and initial contact of the symbionts. We used Suppression Subtractive Hybridization to find up-regulated genes between the green alga Asterochloris sp. and the fungus Cladonia grayi in in vitro resynthesis. We sequenced over 2000 algal and fungal clones, and used BLAST and FASTA algorithmic searches of protein and conserved protein domain databases to characterize our sequences. This dataset represents the first global survey of algal and fungal gene sequences involved in lichen symbiosis, and a summary of these genes and their putative functions will be presented. The results of this study allow us to identify candidate genes of early lichen development for future research.
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1 - Duke University, Department of Biology, 139 Biological Sciences Building, PO Box 90338, Durham, North Carolina, 27708, USA
2 - Duke University, Molecular Genetics and Microbiology, Institute for Genome Sciences and Policy, 289 CARL Building, Research Drive, Durham, NC, 27710, USA
3 - Duke University, Department of Biology, 139 Biological Sciences, Science Dr., Box 90338, Durham, NC, 27708, USA
Presentation Type: Poster:Posters for Topics
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM