Sandoval, Francisco J , Roje, Sanja .
Metabolism of flavin nucleotides in plants.
FMN and FAD are phosphorylated derivatives of riboflavin that are synthesized by the enzymes riboflavin kinase (EC 220.127.116.11) and FAD synthetase (EC 18.104.22.168) in the presence of ATP and Mg2+. These flavin nucleotides are the cofactors for scores of enzymes that participate in vital metabolic processes in all organisms. Despite the vital roles of FMN and FAD in metabolism, much remains to be learned about the enzymes that synthesize and hydrolyze these cofactors in plants. Our long-term goal is to uncover how plants maintain intracellular levels of FMN and FAD.
Toward the goal of advancing basic understanding of FMN and FAD homeostasis in plants, we are now investigating riboflavin kinases, FAD synthetases, FMN hydrolyses and FAD pyrophosphatases from Arabidopsis and pea. Our results suggest that plants contain sequence homologs of riboflavin kinases from bacteria and yeast, and that they contain a novel type of riboflavin kinase in organelles. Our results also show that plant organelles contain FMN hydrolase and FAD pyrophosphatase activities. Here we present our progress in studying enzymes involved in synthesis and hydrolysis of flavin nucleotides in plants.
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1 - Institute of Biological Chemistry, WSU, Pullman, WA, 99164, USA
2 - Institute of Biological Chemistry, WSU
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM