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Abstract Detail


Cell Walls

Shipp, Matthew [1], Nadella, Ramya [1], Faik, Ahmed [2].

Glycoarray, a Novel Technology for Screening of Plant Cell Wall Glycosyltransferase Activities.

The plant cell wall is a well-organized network of polysaccharides with varying degree of complexity. The diversity in the linkages connecting all monosacchatides within these polymers indicates the need for a diversified set of glycosyltransferases to catalyze these glycosidic linkages. The development of a methodology that allows the investigation of the activity of these glycosylatransferase at any given time is required to determine their potential role in building cell walls. Microarrays offer the possibility for gene expression and profiling of glycosyltransferase genes, however, no tool is currently available to study a set of glycosyltranferase activities at the same time. The goal of this study is to develop an easy and efficient way to investigate the activity of several glycosyltransferase activities simultaneously and in a high throughput manner using glycochip technology. Using AtXT1 and AtFUT1, two well characterized glycosyltransferases involved in xyloglucan biosynthesis, we optimized [14C]Xyl and [14C]Fuc incorporation onto different acceptors. Several acceptors, including oligosaccharides and polysaccharides, were tested at various concentrations. The radiolabel can be detected and quantified using a simple high resolution phosphoimager. We were able to quantify as little as 90cpm [14C]-radiolabel incorporation on the slides on 1mm square spots. Our data suggest that glycochip technology can be adapted to glycosyltransferase assays and represent a promising methodology for high throughput screening of new glycosyltransferase activities.


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1 - Ohio University, Environmental and Plant Biology
2 - Ohio University, Environmental and Plant Biology, 512 Porter Hall, Athens, OHIO, 45701, USA

Keywords:
glycochip
glycosyltransferase
cell wall.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P17025
Abstract ID:1112


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