| Abstract Detail
Heavy Metals and Phytoremediation Todorova, Dessislava [1], Miteva, Lyuba [2], Ivanov, Sergei [3], Alexieva, V. [3], Tsekova, K. [1]. Role of glutathione and sulfhydryl groups for cadmium tolerance of Aspergillus niger B77. The aim of this study is better understanding of the role of glutathione, glutathione S-transferase and sulfhydryl groups for the resistance of Aspergillus niger to cadmium ions. Experiments with tolerant strain of Aspergillus niger B77 were conducted. Fungus was treated with 3 different concentrations of Cd ions (260 μM, 444 μM and 620 μM). Experiments were conducted with biomass taken at the middle as well as at the end of the exponential growth phase (12th and 18th hour respectively). Experiments showed that augmentation of Cd ions concentration provoked remarkable reduction of the biomass formation with respect to the control. On the other hand it was found that Cd ions significantly increased glutathione S-transferase activity. Elevation of total and oxidized glutathione content and total sulfhydryl groups was also observed. These data provide evidence for the possible role of sulfhydryl groups, glutathione and glutathione S-transferase in the heavy metal detoxification and in the processes of cadmium resistance of this fungus.
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1 - Institute of Microbiology “Stephan Anegeloff”, Department of Microbial Ecology
2 - Institute of Plant Physiology "Acad. M. Popov", Regulation of Plant Growth and Development, "Acad. G. Bonchev" Str., Bl 21, Sofia, 1113, Bulgaria
3 - Institute of Plant Physiology, Regulation of Plant Growth and Development
Keywords:
A. niger
Heavy Metals
glutathione
sulfhydryl groups.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P05019
Abstract ID:1111 |
