Growth and Vegetative Development
Finer, John , Chiera, Joseph , Bouchard, Robert .
Characterization of 6 different soybean promoters using GFP expression in transgenic soybean.
Six different promoters from soybean (Glycine max Merrill) were isolated, fused to the gfp coding region and reintroduced into soybean. Use of a soybean polyubiquitin (Gmubi) promoter gave the highest and most constitutive expression of GFP in transgenic soybean. High levels of expression were seen in most plant parts with exceptionally high levels of expression in root tissues. Introduction of the Gmubi promoter without the leading intron resulted in lower levels of expression and a slightly different expression pattern. Using microarray data, three other promoters (actin, HSP90-like, and ribosomal S11) were isolated following selection of ESTs which showed preferential expression during soybean somatic embryo induction. All three of these promoters displayed high activity during induction of somatic embryos from immature cotyledons and also directed expression in root tips. Truncation of the 5’ end of the HSP90-like promoter resulted in a stepwise decline in promoter strength, suggesting the presence of regulatory elements in the -628 to -830 region and in the -231 to -443 region. The early embryo expressing actin promoter showed the highest levels of root expression of the three early embryo promoters. A soybean extensin promoter, which was expected to show root-specific expression, was the least active promoter, displaying very low levels of expression only in transgenic soybean root tips. Lastly, a phytase promoter also showed developmentally-regulated expression with activity in hypocotyls of developing soybean somatic embryos.
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1 - OARDC/The Ohio State University, Department of Horticulture and Crop Science, 1680 Madison Ave., Wooster, OH, 44691, USA
2 - OARDC/The Ohio State University, Department of Horticulture and Crop Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM