Systematics Section / ASPT
Cameron, Kenneth M. .
DNA Barcoding The New York Botanical Garden Forest: A Floristic Approach to Plant DNA Barcoding.
A floristically-oriented, plant DNA barcoding project was initiated in order to test five candidate gene regions from the plastid genome (matK, accD, rpoB, rpoC1, and ndhJ) for their applicability in serving as universal plant DNA barcodes. The project was designed to be conducted in parallel with and to complement a phylogenetically-oriented project undertaken by a subset of the Plant Working Group of the Consortium for the Barcode of Life (CBOL). Attempts were made to collect and sequence multiple individuals of every native/naturalized species of vascular plant within the 50-acre forest of The New York Botanical Garden, Bronx, New York for each of the candidate genes. This flora (approximately 343 species in 246 genera from 98 families) represents 8% of all species documented in Gleason and Cronquist’s Manual. As a “real world” test of the five candidate genes, 12 blind samples were collected and sequenced for each gene. The identity of these unknowns was only revealed after the DNA barcode libraries were created. Two alternative search methods were employed to make identifications: a dendrogram building method based on ClustalX alignments with neighbor joining, and a BLASTn search method. Of the five genes studied, matK exhibited the greatest level of sequence variation, and this was more than sufficient to correctly identify most of the unknown samples. In fact, this was true for all of the test genes. Misidentifications resulted only when a lack of pcr product resulted due to primer mismatch for some non-flowering plants. Whereas DNA barcoding of particular taxonomic groups (e.g., palms) may be problematic, this “proof-of-principal” project demonstrated that floristic approaches to plant DNA barcoding hold great promise as an alternative means of plant identification.
Log in to add this item to your schedule
NYBG DNA Barcoding
Natural History Magazine March2007
1 - New York Botanical Garden, Cullman Program for Molecular Systematics Studies, 200th Street and Kazimiroff Boulevard, Bronx, New York, 10458, USA
DNA Bar coding
Presentation Type: Oral Paper:Papers for Sections
Location: Continental C/Hilton
Date: Tuesday, July 10th, 2007
Time: 11:45 AM