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Abstract Detail


Photosynthesis(Carbon)

Kumar, Anshuman [1], Li, Cishan [2], Portis, Archie, R [1].

The rate of Photosynthesis remains relatively high at moderately high temperatures in Arabidopsis thaliana rca mutant expressing thermostable chimeric Rubisco Activase.

The rate of photosynthesis declines at moderately high temperatures (30-42˚C) in temperate plants like Arabidopsis. The decline is due to deactivation of Rubisco which in turn is due to a reduced ability of activase to activate Rubisco (Crafts-Brandner and Salvucci, PNAS 97:13430-13435, 2000). We created a more thermostable activase for Arabidopsis by replacing the Rubisco recognition domain in the more thermostable tobacco activase with that from Arabidopsis. In vitro studies confirmed that the chimeric activase is more thermostable than the native Arabidopsis activase. We then transformed the Arabidopsis rca mutant and selected lines with high expression of this chimeric activase. The initial studies indicate that the rate of photosynthesis as measured by gas exchange is higher than the wild type in the transgenic lines at both 30 and 38˚C. The recovery of photosynthesis is also better in the transgenic lines when the plants are returned to lower temperature (23˚C).


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1 - United States Department of Agriculture, Agricultural Research Service, 193 ERML, 1201 W Gregory, Urbana, IL, 61801, USA
2 - Institute of Human Virology, Baltimore, MD, USA
3 - United States Department of Agriculture, Agricultural Research Service, 193 ERML, 1201 W Gregory, Urbana, IL, 61801, USA

Keywords:
activase
Photosynthesis
Arabidopsis
high temperature.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P13007
Abstract ID:1063


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