Growth and Vegetative Development
Benega Garcia, Reinerio , Tel-Zur, Noemi .
Production of Embryoids in the Tetraploid Selenicereus megalanthus (Vine Cacti) Using Anther and Ovule Culture Techniques.
The night-blooming vine cacti Selenicereus megalanthus (Schum. ex Vaupel) M. is native to Peru, Colombia, Ecuador and Bolivia and is cultivated in Colombia and Israel as an exotic fruit crop. S. megalanthus is tetraploid, with low pollen viability (50-70%), high rate of ovule failure (>77%) and self-fruitfulness. Cytological and molecular studies as well as vegetative and fruit morphology indicated allopolyploid (Hylocereus-Selenicereus) origin. The main goal of this project is to produce allohaploid plants from the tetraploid S. megalanthus using anther and ovule culture. The comparative morphology of these new plants with S. megalanthus and Hylocereus and Selenicereus spp., as well as their chromosome behavior and crossability, will allow us to elucidate the diploid donors of the S. megalanthus genome. Anthers and ovules were collected from flower buds containing microspores at the middle uninucleate developmental stage. Microspores were cultured in vitro on MS media with Picloram/BAP and Thidiazuron (TDZ). The formation of androgenic pro-embryoids started after three days of in vitro culture. Androgenic embryoids developed into plantlets after being transferred to MS culture media in light. Ovules were cultured in vitro on MS media, with GA3, TDZ and sucrose, in dark. Cultured ovules increased in size, then developed into gynogenic embryoids which were transferred to GA3/TDZ media and grown in light to produce plantlets. Subsequent transfers were made to MS medium. Currently, the plantlets originating from androgenic and gynogenic embryoids are growing well and are being hardened off. Chromosome counts, FAC analysis and morphology are currently in process. This study opens new perspectives in the study of evolution, genetics and breeding in vine cacti species.
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1 - Ben-Gurion University, Dryland Biotechnologies, The Institutes for Desert Research
2 - Ben-Gurion University of the Negev, Dryland Biotechnologies, The Institutes for Desert Research, Sde Boqer, Sde Boqer, 84990, Israel
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM