Growth and Vegetative Development
Chettoor, Antony , Xueyuan, Cao , Becraft, Phil .
The COP9 signalsome is involved in ACR4 receptor turnover.
The maize CRINKLY(CR4) gene encodes a receptor-like kinase that is involved in a array of developmental processes, including cell differentiation, cell proliferation, cell fate determination and pattern formation. ACR4 is believed to be the Arabidopsis CR4 orthologue with 60% amino acid identity and all the characteristic motifs of the maize CR4. A yeast two-hybrid screen was used to isolate putative downstream targets of ACR4. Six proteins that interact with the cytoplasmic domain of ACR4 were identified. They include the Cop9 Signalsome Subunits 5A and B (CSN5A, CSN5B), a putative protein phosphatase 2A regulatory subunit B delta (PP2A-Bíδ), a putative lipase and two leucine-rich repeat receptor like kinases (LRR-RLKs). Pull-down assays confirmed interactions between these proteins and ACR4 in-vitro. In-vitro kinase assays demonstrated that ACR4 could phosphorylate CSN5A, CSN5B, and the two RLKs, but the lipase and PP2A-Bíδ were only weakly phosphorylated. In-vivo FRET experiments (Acceptor photobleaching method) demonstrated that the ACR4 receptor was in close proximity to CSN5A, CSN5B and PP2A within the context of a plant cell. Treatment of plants with Curcumin, a COP9 signalsome inhibitor, resulted in the accumulation of ACR4-GFP in plant cells. Genetic experiments to confirm the involvement of the Cop9 signalsome in the regulation of ACR4 are currently in progress.
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1 - Iowa StateUniversity, Genetics,Development and Cell Biology
2 - Iowa State University, Genetics, Development and Cell Biology, Osborne Drive, Ames, IA, 50011, USA
3 - Iowa State University, Department of Genetics, Development and Cell Biology, Ames, Iowa, 50011, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM