Mellon, J. E. , Boue, S. , Shih, B. , Cotty, P. J. .
Compositional Analysis of Sclerotial Extracts from Divergent Fungi within Aspergillus Section Flavi.
Aflatoxin-producing Aspergillus species can be opportunistic fungal seed pathogens of oilseed crops. During crop infection, these fungi frequently produce the potent carcinogen aflatoxin. Aflatoxin contamination of cotton, corn, peanuts or tree nuts can produce significant reductions in crop value. The fungus can over winter in soil environments by means of thick-walled, dark-colored sclerotia. Sclerotia walls typically contain high concentrations of the complex fungal pigment melanin. Since several secondary metabolites are known to accumulate in sclerotia, an investigation was undertaken to determine if sclerotial pigment analysis could distinguish fungal strains, as well as give indications of adaptive differences in accumulation of sclerotial constituents. The analyzed sclerotia were produced in a chemically defined medium by a collection of aflatoxin-producing Aspergillus diverse in both the morphology and pigmentation of sclerotia. Sclerotial walls were pulverized by grinding in a mortar & pestle with liquid nitrogen, followed by extraction with 80% aqueous methanol. Extracts were analyzed by HPLC coupled with UV spectrophotometry or mass spectrometry. Preliminary data suggests both significant qualitative and quantitative differences in metabolites are prevalent within the sampled sclerotia. These results will be discussed.
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1 - USDA, ARS, SRRC, P. O. Box 19687, New Orleans, LA, 70179, U.S.A.
2 - USDA, ARS, University of Arizona, Plant Pathology, 1140 E. South Campus Dr., Tucson, AZ, 85721
Aspergillus section Flavi
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM