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Abstract Detail


Metabolism

Bernal-Gracida, Lilia Angélica [1], Martinez-Barajas, Eleazar [2].

Phosphate deficiency reduces starch breakdown in beans (Phaseolus vulgaris L.).

Phosphorus is essential for living organisms because is a key component in metabolic processes. In response to phosphate (Pi) deficiency plants show biochemical and molecular changes. Our results indicated that after three weeks of Pi starvation, mature leaves of beans (Phaseolus vulgaris L.) accumulated starch. Starch is stored in leaves during the day and then broken down at night. There have been major advances in the understanding of starch degradation in leaves in the last few years. Recent data from studies in Arabidopsis and potato show the importance of ß-amylase in the degradation of the granule. In plants with Pi deficiency the extent of starch breakdown is reduced even though the ß-amylase activity increases. Glucan phosphorylation allows or facilitates starch breakdown and if starch granules are not phosphorylated then they can not be degraded. This work will show that starch granules produced by Pi deficient plants are less phosphorylated, they contain more amylose and its susceptibility to ß-amylase hydrolysis is reduced. Pi deprivation also modifies the interaction between starch granules and proteins. Research supported by FQ-UNAM PAPIIT IN202206, PAIP 6290-14 and CONACyT scholarship to BGLA.


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1 - Universidad Nacional Autónoma de México, Facultad de Química, Departamento de Bioquímica, Conj E Fac de Quimica Circuito Institutos s/n, Universidad 3000 Copilco, Mexico City, DF, 04510, México
2 - Universidad Nacional Autónoma de México, Facultad de Química Departamento de Bioquímica

Keywords:
starch excess
phosphorus deficiency
beta-amylase.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P19025
Abstract ID:1030


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