Padilla-Chacón, Daniel , Martinez-Barajas, Eleazar .
Expression of sugar transporter HXT2 from Saccharomyces cerevisiae in Arabidopsis thaliana leads to modificate the metabolism and distribution of photosynthates in whole plant.
The relation between sink and source organs is important for plant growth and development. The distribution of photosynthates among different sink organs is a complex process regulated at different levels. In several important sink organs photosynthates are unloaded to the apoplastic space, where sucrose is hydrolyzed to glucose and fructose by cell wall invertase. In those organs, the incorporation of resulting hexoses will depend on the activity of monosaccharide transporters located in cell membrane. The expression and activity of several monosaccharide transporters are developmentally regulated. It suggest that hexose transporter are important in regulating photosynthates distribution. In order to enhance sink strength and modificate the distribution of photosynthates, we made a Arabidopsis thaliana transgenic plants that express a hexoses transporter from Saccharomyces cerevisiae (HXT2) under the control of a constitutive promoter (3X35S). Transgenic plants can take more glucose from the medium, roots are longer than in wild type and the activity of cell wall invertase is reduced. The data presented here demonstrate that the hexose uptake can modulate carbohydrate metabolism and the sink-source relationships. Research supported by grants from CONACyT (46827) and PAIP (6290-14), CONACyT provided scholarship (167265) to DPCh.
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1 - Universidad Nacional Autónoma de México, Departamento de Bioquímica, Circuito Institutos s/n Conjunto E, Facultad de Química. Laboratorio 102.
2 - Universidad Nacional Autónoma de México, Departamento de Bioquímica
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM