Jagadish SV, Krishna , Raveendran, Muthurajan , Craufurd, Peter Q , Wheeler, Tim R , Bennett, John .
Understanding the molecular changes in rice spikelets during heat and water stress: a proteomic approach.
Under future climatic scenarios, rice (Oryza sativa L.) will be exposed more frequently to episodes of drought and high temperature, as well as increasing mean temperatures. When these conditions occur at flowering, seed set is greatly reduced. In this study, N22, a drought and heat tolerant genotype, was used to examine the proteomic changes in spikelets exposed to water and heat stress at flowering. Plants were grown in saturated soil culture under optimum conditions of 29o/21oC until 3 days before heading (DBH). Plants were subjected to four treatments: (i) control (optimum conditions); (ii) high temperature (38oC) at heading from 0900 till 1500 h; (iii) water stress starting at 3DBH and (iv) combination of (ii) and (iii) with high temperature on the final day. Flag leaf relative water content was used to quantify the level of water stress. Two-dimensional gel electrophoresis was used to identify the proteins that are affected/induced in the spikelets collected from top four rachis of treated and control panicles. First dimensional IEF using 4-7 pH IPG strips and second dimensional SDS-PAGE gels of 12%, on visualization with silver stain resulted in 1032 spots. Out of 1032 spots, 25 were differentially expressed, with 11 spots significantly changing under water and/or heat stress. The 11 spots were identified by MALDI-TOF to produce protein mass fingerprinting data. The proteins were identified by database searches and the functions identified. Pollen allergens, small molecular weight heat shock proteins and beta expansinís differential expression and their contribution to tolerance under water stress , heat stress and their concurrent occurrence is discussed.
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1 - University of Reading, Agriculture, Plant environment laboratory, cutbuch lane, shinfield road, Reading, Berkshire, RG2 9AF, United Kingdom
2 - International Rice Research Institute, Philippines, PBGB
3 - University of Reading, Agriculture policy and development
Presentation Type: Oral Paper:Papers for Topics
Location: Williford C/Hilton
Date: Wednesday, July 11th, 2007
Time: 8:45 AM